El efecto de la vitamina D3 y su relación. con el nivel de glóbulos blancos en las mujeres con aborto espontáneo, inyección intracitoplasmática de espermatozoides (ICSI) técnicao / The effect of vitamin D3 and its relationship with the level of white blood cells in women spontaneous miscarriage undergoing intracytoplasmic sperm injection (ICSI) technique

Women were studied undergoing ICSI for 84 who suffer non-pregnancy at the Fertility Center, Al-Sadr Medical Hospital in Najaf Governorate, Period between January 2019 and March 2020. WBC, Vitamin D3 and ß-hCG were measured, The pregnant women was divided into (Pregnancy Group, and spontaneous miscarriage) and then demonstrate the immunological effect on pregnancy of women after ICSI technique. Current resultsstudy showed a significant increase (p<0.05) in hormone level ß-hCG is evidence of the presence of high success rates for pregnancy in women who performed operations IVF, where the success rate at the beginning of the matter reached 61.9%, after which it decreased to 33.3% after the first three months due to the occurrence of spontaneous miscarriage of pregnant women due to various immunological and physiological reasons, a positive correlation between the level of ß-hCG and other parameters in the study (Vitamin D3 -WBC).Also The current resultsshowed a significant decrease in a groups (pregnancy failure) and the group (spontaneous miscarriage) compared with the control group (continued pregnancy) in relation to the level of vitamin D3 Also, The current results showed a significant increasein (pregnancy failure) and (spontaneous miscarriage) compared with control groups (continuation of pregnancy) in relation WBC numbers, and the present study founds a negative relationship between the level of vitamin D3 and WBC.

Inhibitory effect of antibodies against human chorionic gonadotropin on the growth of colorectal tumour cells.

Human chorionic gonadotropin (hCG) was initially believed to be secreted exclusively by the embryo with its primary function being “rescue” of the corpus luteum. However, recently it has been found that the hormone (or its individual subunits) is also secreted by many cancers and that in many cases secretion is associated with poor patient prognosis. In this study, we assessed the presence of hCG in colorectal cancer cells (CCL-253) and evaluated the anti-tumour effects of anti-hCG antibodies in vitro and in vivo. Anti-hCG antibodies were reactive with CCL-253, as revealed by confocal immunoflourescence microscopy; both cell surface and intracellular expression were observed. Western blot analysis showed that antibodies appeared to interact with several moieties, indicating a level of cross-reactivity. Anti-hCG antiserum specifically reduced the viability of tumor cells and the addition of complement increased in vitro anti-tumor effects. In nude mice implanted with CCL-253 cells, administration of anti-hCG antiserum caused a significant reduction in tumor volume; all treated animals survived, while mortality was observed in control animals. Results suggest that anti-hCG antibodies can mediate significant anti-tumor activity both in vitro and in vivo and lend support to the rationale of anti-hCG immunization in the therapy of gonadotropin- sensitive cancers.

Participación de hormonas y factores reguladores de la implantación y el desarrollo de la unidad fetoplacentaria / Hormonal participation and regulating factors of implantation and the fetoplacentary unity development

Si bien el embrión tiene un programa genético de su propio desarrollo, para que se lleven a cabo el desarrollo y diferenciación embrionaria, así como la gestación normal, deben establecerse una serie de interacciones coordinadas entre el concepto y la madre, las cuales son mediadas por mensajeros químicos mediante mecanismo autocrinos, paracrinos y endocrinos. En el presente trabajo se analiza la participación de hormonas y factores réguladores de la implantación y el desarrollo de la unidad feto-placentaria

Comportamiento clínico del carcinoma vesical acompañado de producción ectópica de gonadotropina coriónica / Clinical behavior of bladder carcinoma associated with ectopic productions of chorionic gonadotropin

De marzo a septiembre de 1992 se estudiaron 25 pacientes portadores de cáncer vesical de células transicionales, a los cuales se les determinó hormona gonadotropina coriónica en las muestras de patología con técnica de inmunoperoxidasa indirecta. La edad promedio fue de 62 años (24 a 89); 21 eran varones y cuatro mujeres (proporción 5:1). Fumaban 80 por ciento, había hematuria en 76 por ciento y manifestaban síntomas irritativos urinarios bajos 28 por ciento. Del total 21 tenían carcinomas de etapa A y cuatro de etapa B. El promedio de seguimiento fue de 35 meses. De las 25 muestras analizadas, seis (24 por ciento) fueron positivas a la hormona gonadotropina coriónica: la positividad era leve en dos, moderada en dos e intensas en otras dos. Se observó grado de diferenciación III de Ash en 66 por ciento de los casos positivos y en 10 por ciento de los negativos. Seconcluye que la expresión ectópica de la hormona gonadotropina coriónica por tumores vesicales es un factor de mal pronóstico que indica mayor desdiferenciación celular

Desenvolvimento de um ensaio imunofluorométrico para a medida da molécula inteira da gonaatrofina coriônica (hCG) no soro / Development of an immunofluorometric assay for serum measurement of the whole molecule of human chorionic gonatropin (hCG)

Tendo como base dois anticorpos monoclonais produzidos em nosso laboratório, um específico contra a subunidade beta de hCG/LH (E2P1) e outro específico contra um epítopo descontínuo presente na molécula inteira da hCG (F1P16), desenvolvemos um ensaio imunométrico específico para a molécula inteira de hCG. O monoclonal E2P1 foi adsorvido a placas de microtitulaçåo e o monoclonal F1P16 foi marcado com Európio; o ensaio consta de duas incubaçöes sequenciais de 1h cada e leitura final de fluorescência em fluorômetro tempo-sincronizado. A sensibilidade calculada foi de 1,8 UI/L; o perfil de imprecisåo mostrou erro inferior a 19 por cento entre os valores de 5 a 3000 UI/L; o coeficiente de variaçåo interensaio mostrou-se de 8 por cento para uma amostra de valor médio de 1065 UI/L (n=15) e de 13,9 por cento para outra de valor médio de 28 UI/L (n=15). A comparaçåo com método equivalente (Delphia hCG) nåo mostrou nenhuma discordância em 300 amostras dosadas sequencialmente, sendo que em 1388 amostras onde os valores eram iguais ou superiores a 20 UI/L pelo Delfia, obtivemos alto índice de correlaçåo (r=0,988). Os valores obtidos pelo nosso método mostraram-se discreta, porém, significativamente mais elevados, com medianas de 4610 e 4370 UI/L, respectivamente

Desarrollo de métodos inmunologicos para regular la fecundidad

A new approach to fertility regulation is the development of vaccines directed against human substances required for reproduction. Potential candidates for immunological interference include reproductive hormones, ovum and sperm antigens, and antigens derived from embryonic or fetal tissue. Several vaccines targeted at the beta chain of the human chorionic gonadotrophin molecule have reached the clinical trial state in Australia, Finland, India and Sweden, and the preliminary results are very encouraging. A prototype vaccine using the beta subunit of ovine luteinizing hormone seems effective in female primates. Contradictory effects on spermatogenisis in male primates have been observed by different researchers using follicle-stimulating hormone as an antigen. Both this and gonadotrophin-releasing hormone require further basic research. Immunological interference could also be aimed at a sperm production or maturation, or at sperm-ovum interaction in the female reproductive tract. The best characterized sperm antigen to date is LDH-C4, an isoenzime of lactic dehydrogenase. The identification of new sperm antigens is being aided by monoclonal antibody techniques. Another approach is to detect, and then mimic, the antisperm antibodies found in women and men with immunologically mediated infertility. Research on ovum antigens is focusing on the zona pellucida (ZP); anti-ZP antibodies